My one month stay was realized in B.S.N. Srl Biological Sales Network, in the research and development department of the company. A main aspect of my secondment involved in-depth learning of liquid chromatography-tandem mass spectrometry (LC/MS-MS)LC/MS-MS, a powerful analytical tool widely used for its sensitivity, specificity, and ability to quantify and identify multiple compounds within various biological samples. I was introduced to both the theoretical and practical applications of the LC/MS-MS technique. This included an overview of the technology’s key components, such as the LC and MS interfaces, ionization methods, and fragmentation patterns.
I also got instruction about the development of LC/MS-MS analytical methods tailored to the specific compounds that can be anylyzed by kits produced in B.S.N. Srl, including antibiotics, drugs of abuse, steroids, and various endogenous metabolites, as well as metabolites of dieffrent egzogenous substances related to the ocupational medicine. This process emphasized the importance of optimizing various parameters, such as solvent selection, gradient elution, and ionization settings, to achieve robust, reproducible, and sensitive results. I got the insight into data interpretation and troubleshoot challenges related to selectivity, matrix effects, and instrument calibration. A unique learning opportunity was provided through exposure to small-scale manufacturing processes used at B.S.N. Srl. I observed and participated in the preparation of reagents, quality control processes, and packaging stages. B.S.N. Srl focuses on producing high-quality LC/MS-MS kits that simplify sample preparation, thereby improving the overall reliability of analyses for external laboratories. Through this experience, I gained a better understanding of the steps required to maintain batch-to-batch consistency, which is essential for producing kits that meet strict quality standards. I also learned about the importance of internal quality assurance protocols in manufacturing and how these help ensure that the final products meet the high standards required by clients and regulatory bodies. Lyophilization, or freeze-drying, is a crucial process for stabilizing biological and chemical compounds within LC/MS-MS kits. At B.S.N. Srl, I learned about the technical aspects of lyophilization, which involves freezing the product and reducing the surrounding pressure to remove water by sublimation. This technique is essential in enhancing the stability and shelf-life of sensitive reagents, such as biological markers or calibration standards.
In summary, my secondment at B.S.N. Srl was a valuable experience that broadened my technical expertise and provided me with practical insights into the commercial aspects of LC/MS-MS kit production. I also got a broader understanding of the possibilities for collaboration between private sector companies and academic institutions. Working closely with the team at B.S.N. Srl highlighted how the practical expertise and resources of the private sector can effectively complement academic research, creating opportunities for meaningful partnerships that drive both innovation and knowledge exchange.
Dr Jelena Savić and Dr Milica Zrnić, Faculty of Pharmacy, University of Belgrade
