“During my professional engagement at the University of Crete Medical School in Heraklion, I had the privilege of working closely with Professor Christos Tsatsanis and Professor Dimitris Kardassis. In the university’s research laboratory, I gained hands-on experience with advanced molecular biology techniques, including RNA extraction and the assessment of RNA quality and quantity using RT-qPCR technology.

Thanks to Professor Christos Tsatsanis, I also had the chance to observe operations in a clinical laboratory. As the head of the biochemistry and immunochemistry departments, I had an in-depth look at the structure and workflow of their lab operations.Through discussions with Professor Tsatsanis and his lab team, I realized that the laboratory where I work is on par with theirs in terms of equipment, work quality, and adherence to laboratory standards.In summary, this experience was invaluable, as it expanded my knowledge of molecular biology techniques. Working in a research laboratory provided me with a better understanding of the complexities of scientific research. Additionally, collaborating with motivated young researchers proved that while challenging, such efforts produce long-term, meaningful results. Moreover, the professionalism, warmth, and exchange of ideas I experienced reaffirmed the notion that science transcends borders. Cooperation and communication among colleagues and researchers are key to progress.

I am deeply thankful for the opportunity to meet and collaborate with such exceptional professionals during my time in Heraklion”

Dr Iva Perović Blagojević, Clinical-Hospital Center “Dr Dragiša Mišović – Dedinje” 

“During my stay in Heraklion, I was at the University of Crete, where I collaborated with Professors Christos Tsatsanis and Dimitris Kardassis. In the research laboratory located at the University, I was introduced with advanced molecular biology techniques. This included execution of RNA extraction procedures, evaluation of its quality and quantity, as well as real-time PCR reactions. 

By courtesy of the professor Tsatsanis I also had the opportunity to visit a clinical laboratory. It was a particular pleasure visiting the clinical laboratory considering the fact that I work in one at my core hospital. I managed to get insight with the work process and organization of the laboratory, as well as to exchange valuable experiences with colleagues. Besides allowing me a broader insight related to my daily work I was greatly pleased to conclude that the laboratory where I work matches the quality of equipment of such a renowned laboratory. 

This experience was valuable for me, allowing me to take a broader view of my professional role in the clinical laboratory where I work.Moreover, I had the pleasure of meeting wonderful people in the clinical as well as in the resarch laboratory who were more than willing to share their expertise in a collegial manner. Their expertise, responsibility, and desire to share knowledge greatly contributed to my sense of belonging to their collective. 

I am immensely grateful for the opportunity to meet, collaborate, and spend time with people who are exceptional both professionally and personally. “

Jovana Milijić Jovanović, Clinical-Hospital Center “Dr Dragiša Mišović – Dedinje”

”During my secondment at the Medical School in Crete from September 15, 2024, to October 16, 2024, I had the privilege of combining in-depth research with valuable learning experiences. 

The secondment started with a Summer School, which provided us with a great opportunity to refresh and expand our knowledge. We went over the basics of epigenetics at the RNA level, which gave me a clearer understanding of how gene expression can be influenced beyond the DNA sequence. We also had workshops that included hands-on programming, which helped us explore bioinformatics tools—a crucial skill in modern biomedical research.After the Summer School, I began working in the lab under the guidance of Professor Christos Tsatsanis, focusing on sample preparation for transcriptomics. I was lucky to collaborate with my peers Maria, Raina, and Elina, and together we tackled various stages of experiments, learning from each other along the way. Our teamwork made the experience not only more productive but also enjoyable, as we shared ideas, challenges, and solutions.

One of the most impactful parts of the secondment for me was the in-depth work with RNA quantification and PCR. To ensure the quality of our RNA samples, we used a NanoDrop spectrophotometer. This small but powerful device measures the concentration and purity of RNA by analyzing how light passes through a small droplet of the sample. Specifically, it measures absorbance at 260 nm (where nucleic acids absorb light) and 280 nm (where proteins absorb). A high 260/280 ratio indicates good purity, while lower ratios suggest contamination. This step was essential because high-quality RNA is critical for reliable downstream experiments like PCR. It was fascinating to see how much precision and care these processes required. Quantifying RNA properly was essential to make sure our samples were of high quality and ready for further analysis. Small mistakes could affect the entire downstream process, so we had to be thorough.

Running PCR (Polymerase Chain Reaction) to amplify specific RNA sequences was equally eye-opening. It showed me how a small amount of RNA could be amplified to detect and study specific genes. I learned how to troubleshoot when things didn’t go as planned—whether it was an unexpected result or a reaction that failed. This hands-on experience really brought home the importance of patience, attention to detail, and consistency in research. Throughout the secondment, Professor Tsatsanis was an incredible mentor. He not only guided us through the technical aspects of our work but also gave us insights into the bigger picture—how each experiment fits into the larger field of transcriptomics. His expertise and encouragement helped me gain confidence in my skills and understanding of complex molecular biology techniques.

Outside the lab, my time in Heraklion was just as memorable. The city’s blend of history, culture, and modern life provided a perfect balance to the intensive work we were doing in the lab. I enjoyed exploring the streets, trying local dishes, and visiting landmarks. It was also great to unwind with my peers, sharing stories over food and enjoying the lively atmosphere of Crete.

Working alongside Maria, Raina, and Elina in Professor Tsatsanis’ lab was an incredible experience. Each of us brought different strengths to the table, and we supported each other throughout the secondment. Our collaboration made even the more challenging parts of the work enjoyable. ”

Aleksa Petković, PhD student, Clinical-Hospital Center “Dr Dragiša Mišović – Dedinje”